rabbit anti col1  (Bioss)

Journal: Bone & Joint Research

Article Title: High-intensity running exercise promotes knee meniscal damage via the PI3K/AKT/mTOR axis

doi: 10.1302/2046-3758.1411.BJR-2024-0535.R1

Figure Lengend Snippet: Excessive mechanical stress promotes meniscus degeneration.a) Immunohistochemical staining revealed the expression of Aggrecan and matrix metalloproteinase 13 (MMP13) in the anterior horn, posterior horn, and articular surface of the meniscus following treadmill intervention in mice. Scale bar: 100 µm. (The same group showed serial sections from the same mouse knee joint tissue for staining analysis.) b) Statistical analysis of the differences in MMP13 and Aggrecan expression between the anterior and posterior horns of the meniscus and the articular cartilage surface groups (n = 5/group). Data were analyzed using one-way analysis of variance (ANOVA) followed by Tukey's post-hoc test. c) Immunofluorescence displaying COL1-positive, Aggrecan-positive, MMP13-positive, and a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5)-positive cells as a proportion of total meniscal cells in primary medial meniscal cells (n = 3/group). Data are presented as the mean (SD). Scale bar: 200 µm. d) Quantification of the positive meniscus cells (P-PI3K, P-AKT, and P-S6) as a proportion of total meniscal cells (n = 3/group). Data were analyzed using independent-samples t -test. *p < 0.05, **p < 0.01, ***p < 0.001. ns, non-significant.

Article Snippet: Mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:10,000, ab8245; Abcam, UK), rabbit anti-MMP13 (1:2,000, 18165-1-AP; Proteintech, USA), rabbit anti-A disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) (1:1,000, A2836; Abclonal, USA), rabbit anti-collagen type I (COL1) (1:2,000, 14695-1-AP; Proteintech), rabbit anti-Aggrecan (1:1,000, A11691; Abclonal), rabbit anti-phospho-PI3K p85 (1:1,000, YP0224; ImmunoWay, USA), rabbit anti-phospho-AKT (Thr308) (1:500, PC2720; Abmart, China), rabbit anti-phospho-S6 (S240/S244) (1:500, AP0537; Abclonal), rabbit anti-PI3K (1:500, A17433; Abclonal), rabbit anti-AKT (1:1,000, ab192623; Abcam), and rabbit anti-S6 (1:500, A6058; Abclonal) served as the primary antibodies used.

Techniques: Immunohistochemical staining, Staining, Expressing, Immunofluorescence

Journal: World Journal of Stem Cells

Article Title: Low-intensity pulsed ultrasound reduces alveolar bone resorption during orthodontic treatment via Lamin A/C-Yes-associated protein axis in stem cells

doi: 10.4252/wjsc.v16.i3.267

Figure Lengend Snippet: The bone density decreased, and the alveolar bone height decreased on the compression side. A: Representative pictures of the rat tooth movement model and 3D model images reconstructed with Mimics software (left) and the statistical diagram of the tooth movement distance (right); B: Representative images of 3D models reconstructed from micro-computed tomography images showing the distance from the alveolar crest to the cemento-enamel junction (left) and the corresponding statistical analysis (right); C: Representative region of interest (ROI) selection diagrams (left) and statistical analysis (right). A certain volume of alveolar bone on the compressed side of the middle 1/2 root of the distal buccal root of the first molar was selected for subsequent analysis. The statistical analysis of the BV/TV, Tb.Th, Tb.N, Tb.Sp and BS/BV values of the ROI region was performed with Siemens software; D: Representative images of hematoxylin and eosin staining; E: Representative images of immunohistochemical staining for type 1 collagen and osteocalcin (left) and quantitative analyses (right); F: Representative images of tartrate-resistant acid phosphatase (TRAP) staining (left) and counting of TRAP-positive multinuclear (> 3 nuclei) cells (right). a P < 0.05 vs control group, b P < 0.01 vs control group. CEJ: Cementum-enamel boundary; HE: Hematoxylin and eosin; TRAP: Tartrate-resistant acid phosphatase; COL1: Type 1 collagen; OCN: Osteocalcin; CT: Computed tomography.

Article Snippet: The primary antibodies used were rabbit anti-type 1 collagen (COL1) and anti-osteocalcin (OCN) (1:500; Abcam, Cambridge, Massachusetts, United States), rabbit anti-ALP (1:500; Invitrogen), and mouse anti-GAPDH (1:1000; ProteinTech, Cook, Illinois, United States).

Techniques: Software, Micro-CT, Selection, Staining, Immunohistochemical staining, Computed Tomography

Journal: World Journal of Stem Cells

Article Title: Low-intensity pulsed ultrasound reduces alveolar bone resorption during orthodontic treatment via Lamin A/C-Yes-associated protein axis in stem cells

doi: 10.4252/wjsc.v16.i3.267

Figure Lengend Snippet: Static pressure inhibits the osteogenic differentiation of bone marrow mesenchymal stem cells in vitro . A and B: Representative alkaline phosphatase (ALP) staining images and statistical analysis; C and D: Representative alizarin red staining images and quantitative data from the alirazin red assay; E: mRNA expression of ALP, type 1 collagen (COL1), runt-related transcription factor 2, and osteocalcin (OCN) folded to control group by quantitative reverse transcription-polymerase chain reaction; F: COL1, ALP, and OCN protein expression in bone marrow mesenchymal stem cells analyzed by Western blotting (left), and the band intensities measured with ImageJ software (right). a P < 0.05 vs control group, b P < 0.01 vs control group. ALP: Alkaline phosphatase; RUNX2: Runt-related transcription factor 2; COL1: Type 1 collagen; OCN: Osteocalcin; ARS: Alizarin red staining.

Article Snippet: The primary antibodies used were rabbit anti-type 1 collagen (COL1) and anti-osteocalcin (OCN) (1:500; Abcam, Cambridge, Massachusetts, United States), rabbit anti-ALP (1:500; Invitrogen), and mouse anti-GAPDH (1:1000; ProteinTech, Cook, Illinois, United States).

Techniques: In Vitro, Staining, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Software

Journal: World Journal of Stem Cells

Article Title: Low-intensity pulsed ultrasound reduces alveolar bone resorption during orthodontic treatment via Lamin A/C-Yes-associated protein axis in stem cells

doi: 10.4252/wjsc.v16.i3.267

Figure Lengend Snippet: Low-intensity pulsed ultrasound promotes the osteogenesis of bone marrow mesenchymal stem cells. A and B: Representative alkaline phosphatase (ALP) staining images and statistical analysis; C and D: Representative alizarin red staining images and quantitative data from the alirazin red assay; E: mRNA expression of ALP, type 1 collagen (COL1), runt-related transcription factor 2, and osteocalcin (OCN) folded to control group by quantitative reverse transcription-polymerase chain reaction; F: COL1, ALP, and OCN protein expression in rat alveolar bone tissues analyzed via Western blotting (left), and the band intensities measured via ImageJ software (right). a P < 0.05 vs control group, b P < 0.01 vs control group. ALP: Alkaline phosphatase; RUNX2: Runt-related transcription factor 2; COL1: Type 1 collagen; OCN: Osteocalcin; LIPUS: Low-intensity pulsed ultrasound; ARS: Alizarin red staining.

Article Snippet: The primary antibodies used were rabbit anti-type 1 collagen (COL1) and anti-osteocalcin (OCN) (1:500; Abcam, Cambridge, Massachusetts, United States), rabbit anti-ALP (1:500; Invitrogen), and mouse anti-GAPDH (1:1000; ProteinTech, Cook, Illinois, United States).

Techniques: Staining, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Software

Journal: World Journal of Stem Cells

Article Title: Low-intensity pulsed ultrasound reduces alveolar bone resorption during orthodontic treatment via Lamin A/C-Yes-associated protein axis in stem cells

doi: 10.4252/wjsc.v16.i3.267

Figure Lengend Snippet: Low-intensity pulsed ultrasound promotes bone formation. A: Representative images of hematoxylin and eosin staining; B: Representative images of immunohistochemical staining for type 1 collagen and osteocalcin and tartrate-resistant acid phosphatase staining (TRAP); C-E: Statistical analyses of the immunohistochemical staining and TRAP staining results. a P < 0.05 vs control group, b P < 0.01 vs control group. HE: Hematoxylin and eosin; TRAP: Tartrate-resistant acid phosphatase; COL1: Type 1 collagen; OCN: Osteocalcin; LIPUS: Low-intensity pulsed ultrasound.

Article Snippet: The primary antibodies used were rabbit anti-type 1 collagen (COL1) and anti-osteocalcin (OCN) (1:500; Abcam, Cambridge, Massachusetts, United States), rabbit anti-ALP (1:500; Invitrogen), and mouse anti-GAPDH (1:1000; ProteinTech, Cook, Illinois, United States).

Techniques: Staining, Immunohistochemical staining

Journal: World Journal of Stem Cells

Article Title: Low-intensity pulsed ultrasound reduces alveolar bone resorption during orthodontic treatment via Lamin A/C-Yes-associated protein axis in stem cells

doi: 10.4252/wjsc.v16.i3.267

Figure Lengend Snippet: siYAP1 blocks the effect of low-intensity pulsed ultrasound-induced osteogenic differentiation of bone marrow mesenchymal stem cells. A: Representative immunofluorescence images of Yes-associated protein (YAP1) (red) and DAPI (blue) staining (left) and statistical analyses (right); B: Western blotting results for type 1 collagen, YAP1, runt-related transcription factor 2 (RUNX2), and alkaline phosphatase (ALP); C: mRNA expression of ALP, RUNX2, and YAP1 in the force group by quantitative reverse transcription-polymerase chain reaction; D and E: Representative ALP and alizarin red staining staining images and statistical analysis. a P < 0.05 vs control group, b P < 0.01 vs control group. ALP: Alkaline phosphatase; COL1: Type 1 collagen; LIPUS: Low-intensity pulsed ultrasound; RUNX2: Runt-related transcription factor 2; YAP1: Yes-associated protein; ARS: Alizarin red staining.

Article Snippet: The primary antibodies used were rabbit anti-type 1 collagen (COL1) and anti-osteocalcin (OCN) (1:500; Abcam, Cambridge, Massachusetts, United States), rabbit anti-ALP (1:500; Invitrogen), and mouse anti-GAPDH (1:1000; ProteinTech, Cook, Illinois, United States).

Techniques: Immunofluorescence, Staining, Western Blot, Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: World Journal of Stem Cells

Article Title: Low-intensity pulsed ultrasound reduces alveolar bone resorption during orthodontic treatment via Lamin A/C-Yes-associated protein axis in stem cells

doi: 10.4252/wjsc.v16.i3.267

Figure Lengend Snippet: Putative mechanism of the effect of low-intensity pulsed ultrasound on alveolar bone during orthodontic treatment. Low-intensity pulsed ultrasound has little effect on osteoclast differentiation but may rescue osteogenic gene expression suppressed by static force and promote osteoblastic differentiation by reordering the cytoskeleton, upregulating the expression of F-actin and Lamin A/C, and increasing the nuclear translocation of Yes-associated protein 1, thereby contributing to alveolar bone homeostasis and morphology while accelerating tooth movement during orthodontic treatment. COL1: Type 1 collagen; LIPUS: Low-intensity pulsed ultrasound; RUNX2: Runt-related transcription factor 2; BMSC: Bone marrow mesenchymal stem cell; YAP1: Yes-associated protein.

Article Snippet: The primary antibodies used were rabbit anti-type 1 collagen (COL1) and anti-osteocalcin (OCN) (1:500; Abcam, Cambridge, Massachusetts, United States), rabbit anti-ALP (1:500; Invitrogen), and mouse anti-GAPDH (1:1000; ProteinTech, Cook, Illinois, United States).

Techniques: Expressing, Translocation Assay

Journal: iScience

Article Title: Targeting endometrial inflammation in intrauterine adhesion ameliorates endometrial fibrosis by priming MSCs to secrete C1INH

doi: 10.1016/j.isci.2023.107201

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-Col1 , Proteintech , Cat# 14695-1-AP; RRID: AB_2082037.

Techniques: Staining, Recombinant, Cell Counting, Enzyme-linked Immunosorbent Assay, Western Blot, Software